马思远*，李慕怡，黎 明

（四川文理学院体育学院，达州635000）

摘要：以扛板归为原材料，超声辅助酶法提取扛板归多糖。以多糖得率为评价指标，通过单因素试验与响应面设计优化扛板归多糖的提取工艺，之后进行动物试验考察扛板归多糖对运动性疲劳的影响。结果表明，超声辅助酶法提取扛板归多糖的最佳工艺条件为液料比28∶1mL/g、复合酶用量（m纤维素酶∶m果胶酶∶m木瓜蛋白酶＝1∶1∶1）1.2%、超声功率260 W、酶解温度40 ℃、酶解时间51 min，在此条件下扛板归多糖得率为（7.61±0.03）%。与空白对照组相比，中、高剂量的扛板归多糖可显著延长小鼠的力竭游泳时间和爬杆时间（P＜0.05，P＜0.01），显著降低小鼠运动后血清中乳酸与尿素氮含量（P＜0.05，P＜0.01），并能显著增强体内超氧化物歧化酶和谷胱甘肽过氧化物酶的活力（P＜0.01），表明扛板归多糖具有缓解小鼠运动疲劳的活性。该研究为相关运动食品的开发提供借鉴。

关键词：扛板归；多糖；复合酶；超声提取；抗运动性疲劳；响应面法

中图分类号：TS201.2 文献标识码：A 文章编号：1674-506X（2025）02-0025-0009

Optimization of Ultrasonic-assisted Enzymatic Extraction Process for Polysaccharide from Polygonum perfoliatum L. and Its Effect on Exercise Fatigue

MA Siyuan^*, LI Muyi, LI Ming

(School of Physical Education, Sichuan University of Arts and Science, Dazhou 635000, China)

Abstract: Using Polygonum perfoliatum L. as raw material, the polysaccharides of P. perfoliatum L. were extracted by ultrasonic-assisted enzyme method. With the yield of polysaccharides as the evaluation index, this polysaccharides of P. perfoliatum L. extraction process was optimized by single factor test and response surface design, and through further animal test the anti-exercise fatigue effect of the polysaccharides of P. perfoliatum L. was investigated. The results showed that the optimum process conditions for the extraction of the polysaccharides of P. perfoliatum L. by ultrasonic-assisted enzyme method were as follows: liquid-solid ratio 28∶1 mL/g, complex enzyme amount (mcellulase∶mpectinase∶mpapain＝1∶1∶1) 1.2%, ultrasonic power 260 W, enzymolysis temperature 40 ℃, enzymolysis time 51 min, under these conditions the yield of polysaccharides of P. perfoliatum L. reached (7.61±0.03)%. Compared with the blank control group, the polysaccharides of P. perfoliatum L. in middle or high dose could significantly prolong time of exhausting swimming and pole climbing in mice (P<0.05, P<0.01), significantly decrease the lactic acid and urea nitrogen content in serum of mice after exercise (P<0.05, P<0.01), and remarkably strengthen activity of SOD and GSH-Px (P<0.01) in vivo. These phenomena reveals that the polysaccharides of P. perfoliatum L. have the activity of alleviating mouse exercise fatigue, this research can provide a reference for developing related sports food.

Keywords: Polygonum perfoliatum L.; polysaccharides; complex enzyme; ultrasonic extraction; anti-exercise fatigue; response surface methodology

doi: 10.3969/j.issn.1674-506X.2025.02-004